PM150610P
$ 30.00
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Product Introduction
Background
M199 (Medium 199) originally used in nutritional studies of chicken embryo fibroblasts, it has been widely used in the culture of various animal cells, including some non-mammalian cells. M199 is especially suitable for the culture of untransformed cells, rat pancreatic epithelial cells and mouse lens tissues, also used in virology and vaccine production. Compared with other basic medium, M199 contains unique ingredients, including adenine, adenosine, hypoxanthine, thymine, and other vitamins. There are two kinds of equilibrium salt (earle's salt and hank's salt) in M199. Earle's salt is often used in CO₂ environment, hank's salt is used in non-CO₂ environment.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000 mg/L D-Glucose
• 100 mg/L L-Glutamine
• 20 mg/L Phenol red
• Earle's Balanced salt
Without
• HEPES
• NaHCO₃
Matters Need Attention
1. Please wear a lab coat and use disposable gloves and a mask during operation.
2. To ensure the optimal performance of this product, please strictly adhere to the recommended storage conditions for its preservation.
3. This product is intended for scientific research exclusively or as a raw material in the production process, and must not be applied for clinical diagnosis or treatment.
Specifications
Volume | 5×1L 1×10L 1×50L 100L 500L |
Form | Powder |
Bacteria Detection | ≤ 100 |
Fungi Detection | ≤ 20 |
Endotoxin Content | < 0.25 EU/mL |
pH | 4.4-4.8 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 36 months |
Storage Condition | This product can be stored at 2-8℃ for 36 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2023) IF: 5.6
DOI: 10.1016/j.jnutbio.2023.109436
Product Cited: M199, powder Medium