PM150710
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Product Introduction
Background
McCoy's 5A medium was initially used specifically for the culture of Novikoff Hepatoma cells. Unlike other medium, McCoy's 5A medium contains reduced glutathione, bacterial peptone and high concentrations of glucose. McCoy's 5A is widely used in the culture of primary cells, such as bone marrow, skin, gingiva, kidney, spleen, lung, rat embryo, omentum and so on. In addition, McCoy's 5A medium is also used for tissue biopsy culture, the establishment of cell line, and the culture of some lymphocytes and more difficult cells.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3000 mg/L D-Glucose
• 1.5 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Publications
Journal: JOURNAL OF COLLOID AND INTERFACE SCIENCE (2025) IF: 9.4
DOI: 10.1016/j.jcis.2025.01.177
Product Cited: Human Umbilical Vein Endothelial Cells, HCT 116 Cells Line, McCoy's 5A Medium, U-2 OS [U2OS] Cells Line
Journal: Cell Death & Disease (2025) IF: 8.1
DOI: 10.1038/s41419-025-07673-2
Product Cited: McCoy's 5A Medium, MEM Medium
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2025) IF: 7.7
DOI: 10.1016/j.ijbiomac.2025.142791
Product Cited: McCoy's 5A Medium, DMEM (High glucose) Medium, Phosphate Buffer (PBS, 1 ×), Fetal Bovine Serum
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2025) IF: 7.7
DOI: 10.1016/j.ijbiomac.2025.140057
Product Cited: HT-29 Cells Line, McCoy's 5A Medium, Raji Cells Line
Journal: ONCOGENE (2025) IF: 6.9
DOI: 10.1038/s41388-025-03289-0
Product Cited: RPMI-1640 Medium, DMEM (High glucose) Medium, McCoy's 5A Medium, MEM, with NEAA Medium
