PM150710
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Product Introduction
Background
McCoy's 5A medium was initially used specifically for the culture of Novikoff Hepatoma cells. Unlike other medium, McCoy's 5A medium contains reduced glutathione, bacterial peptone and high concentrations of glucose. McCoy's 5A is widely used in the culture of primary cells, such as bone marrow, skin, gingiva, kidney, spleen, lung, rat embryo, omentum and so on. In addition, McCoy's 5A medium is also used for tissue biopsy culture, the establishment of cell line, and the culture of some lymphocytes and more difficult cells.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3000 mg/L D-Glucose
• 1.5 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH (2025) IF: 1.9
DOI: 10.1590/1414-431X2024e14187
Product Cited: Recombinant Human Interleukin-2 (IL-2) Solution, McCoy's 5A Medium, Penicillin-Streptomycin Solution, 100 ×
Journal: Translational Cancer Research (2025) IF: 1.5
DOI: 10.21037/tcr-24-1397
Product Cited: McCoy's 5A Medium
Journal: Translational Cancer Research (2025) IF: 1.5
DOI: 10.21037/tcr-24-1608
Product Cited: McCoy's 5A Medium
Journal: JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY (2025) IF: 1.5
DOI: 10.1007/s10967-025-10080-8
Product Cited: Fetal Bovine Serum, MEM Medium, Penicillin-Streptomycin Solution, 100 ×, HT-29 Cells Line, S-180 Cells Line, McCoy's 5A Medium, Hela Cells Line
Journal: CANCER LETTERS (2024) IF: 9.7
DOI: 10.1016/j.canlet.2024.216732
Product Cited: McCoy's 5A, DMEM(High glucose), Donor Equine Serum, EGF/Urogastrone/URG, Human, Recombinant, 10 mg/mL Recombinant Human Insulin Solution, MEM Non-Essential Amino Acid Solution (NEAA), 100×, Penicillin-Streptomycin Solution, 100×, RPMI-1640