PM150710
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Product Introduction
Background
McCoy's 5A medium was initially used specifically for the culture of Novikoff Hepatoma cells. Unlike other medium, McCoy's 5A medium contains reduced glutathione, bacterial peptone and high concentrations of glucose. McCoy's 5A is widely used in the culture of primary cells, such as bone marrow, skin, gingiva, kidney, spleen, lung, rat embryo, omentum and so on. In addition, McCoy's 5A medium is also used for tissue biopsy culture, the establishment of cell line, and the culture of some lymphocytes and more difficult cells.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3000 mg/L D-Glucose
• 1.5 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: JOURNAL OF MEDICINAL CHEMISTRY (2025) IF: 6.8
DOI: 10.1021/acs.jmedchem.4c02917
Product Cited: Fetal Bovine Serum, McCoy's 5A Medium
Journal: npj Precision Oncology (2025) IF: 6.8
DOI: 10.1038/s41698-024-00791-8
Product Cited: McCoy's 5A Medium, Penicillin-Streptomycin Solution, 100 ×, RPMI-1640 Medium
Journal: International Journal of Nanomedicine (2025) IF: 6.6
DOI: 10.2147/IJN.S502076
Product Cited: HCT 116 Cells Line, McCoy's 5A Medium
Journal: EMBO REPORTS (2025) IF: 6.5
DOI: 10.1038/s44319-025-00385-w
Product Cited: RPMI-1640 Medium, SK-BR-3 [SKBR3] Cells Line, T-47D Cells Line, HCC1937 Cells Line, BT-549 Cells Line, ZR-75-1 Cells Line, JeKo-1 Cells Line, 293FT Cells Line, MDA-MB-436 Cells Line, Jurkat, Clone E6-1 Cells Line, McCoy's 5A Medium, DMEM (High glucose) Medium, BT-474 [BT474] Cells Line
Journal: JCI Insight (2025) IF: 6.3
DOI: 10.1172/jci.insight.186871
Product Cited: DMEM (High glucose) Medium, MEM, with NEAA Medium, McCoy's 5A Medium, Ham's F-12K Medium