PM150710
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Product Introduction
Background
McCoy's 5A medium was initially used specifically for the culture of Novikoff Hepatoma cells. Unlike other medium, McCoy's 5A medium contains reduced glutathione, bacterial peptone and high concentrations of glucose. McCoy's 5A is widely used in the culture of primary cells, such as bone marrow, skin, gingiva, kidney, spleen, lung, rat embryo, omentum and so on. In addition, McCoy's 5A medium is also used for tissue biopsy culture, the establishment of cell line, and the culture of some lymphocytes and more difficult cells.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3000 mg/L D-Glucose
• 1.5 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
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Publications
Journal: RSC Medicinal Chemistry (2024) IF: 4.1
DOI: 10.1039/D3MD00619K
Product Cited: A549 [A-549] Cells Line, NCI-H460 [H460] Cells Line, PC-9 Cells Line, Calu-1 Cells Line, SK-BR-3 [SKBR3] Cells Line, SK-OV-3 Cells Line, 786-O [786-0] Cells Line, HuH-7 Cells Line, HCT 116 Cells Line, Hela Cells Line, WI-38 Cells Line, MCF 10A Cells Line, KGN Cells Line, 293T [HEK-293T] Cells Line, DMEM(High glucose), MEM, DMEM/F12, McCoy's 5A, RPMI-1640, 0.25% Trypsin Solution
Journal: Aging-US (2024) IF: 3.9
Product Cited: 293T [HEK-293T] Cells Line, RPMI-1640 Medium, DMEM (High glucose) Medium, HT-29 Cells Line, CW-2 Cells Line, Ham's F-12K Medium, McCoy's 5A Medium, Fetal Bovine Serum
Journal: Scientific Reports (2024) IF: 3.8
DOI: 10.1038/s41598-024-78413-3
Product Cited: MDA-MB-231 Cells Line, MDA-MB-453 Cells Line, McCoy's 5A Medium, MCF 10A Cells Line, SK-BR-3 [SKBR3] Cells Line, BT-549 Cells Line, MCF7 [MCF-7] Cells Line
Journal: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2024) IF: 3.8
DOI: 10.1016/j.abb.2024.110128
Product Cited: McCoy's 5A Medium
Journal: DRUG DEVELOPMENT RESEARCH (2024) IF: 3.5
DOI: 10.1002/ddr.22242
Product Cited: McCoy's 5A Medium, J82 Cells Line, Penicillin-Streptomycin Solution, 100 ×, MEM, with NEAA Medium, Fetal Bovine Serum, T24 [T-24] Cells Line
