PM150710
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Product Introduction
Background
McCoy's 5A medium was initially used specifically for the culture of Novikoff Hepatoma cells. Unlike other medium, McCoy's 5A medium contains reduced glutathione, bacterial peptone and high concentrations of glucose. McCoy's 5A is widely used in the culture of primary cells, such as bone marrow, skin, gingiva, kidney, spleen, lung, rat embryo, omentum and so on. In addition, McCoy's 5A medium is also used for tissue biopsy culture, the establishment of cell line, and the culture of some lymphocytes and more difficult cells.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3000 mg/L D-Glucose
• 1.5 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Publications
Journal: CARCINOGENESIS (2024) IF: 3.3
Product Cited: McCoy's 5A Medium, RPMI-1640 Medium
Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY (2024) IF: 3.3
DOI: 10.1016/j.taap.2024.117221
Product Cited: Annexin V-APC/PI Apoptosis Kit, McCoy's 5A Medium, Fetal Bovine Serum, SW480 [SW-480] Cells Line, Penicillin-Streptomycin Solution, 100 ×, HCT 116 Cells Line, DMEM (High glucose) Medium
Journal: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY (2024) IF: 3.2
DOI: 10.1002/bab.2643
Product Cited: SK-OV-3 Cells Line, Penicillin-Streptomycin Solution, 100 ×, Caov-3 Cells Line, RPMI-1640 Medium, DMEM (High glucose) Medium, McCoy's 5A Medium, NIH:OVCAR-3 [OVCAR3] Cells Line
Journal: JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2024) IF: 3.2
DOI: 10.1002/jbt.70103
Product Cited: DMEM (High glucose) Medium, Leibovitz's L-15 Medium, McCoy's 5A Medium, Ham's F-12K Medium
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024) IF: 3.1
DOI: 10.1016/j.bbrc.2024.150176
Product Cited: McCoy's 5A Medium
