PM150710
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Product Introduction
Background
McCoy's 5A medium was initially used specifically for the culture of Novikoff Hepatoma cells. Unlike other medium, McCoy's 5A medium contains reduced glutathione, bacterial peptone and high concentrations of glucose. McCoy's 5A is widely used in the culture of primary cells, such as bone marrow, skin, gingiva, kidney, spleen, lung, rat embryo, omentum and so on. In addition, McCoy's 5A medium is also used for tissue biopsy culture, the establishment of cell line, and the culture of some lymphocytes and more difficult cells.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3000 mg/L D-Glucose
• 1.5 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: JOURNAL OF BIOLOGICAL CHEMISTRY (2023) IF: 4.800
DOI: 10.1016/j.jbc.2023.104621
Product Cited: McCoy's 5A, MEM(with NEAA), RPMI-1640, DMEM(High glucose), Renca [RenCa] Cells Complete Medium
Journal: FASEB JOURNAL (2023) IF: 4.8
Product Cited: McCoy's 5A
Journal: Frontiers in Oncology (2023) IF: 4.7
DOI: 10.3389/fonc.2023.1166444
Product Cited: 293 [HEK-293] Cells Line, HCT 116 Cells Line, DLD-1 Cells Line, McCoy's 5A, Fetal Bovine Serum, RPMI-1640, DMEM(High glucose)
Journal: CARCINOGENESIS (2023) IF: 4.7
Product Cited: T-47D Cells Line, SK-BR-3 [SKBR3] Cells Line, Leibovitz's L-15, McCoy's 5A
Journal: CARCINOGENESIS (2023) IF: 4.7
Product Cited: HCT 116 Cells Line, LS180 Cells Line, McCoy's 5A, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×, MEM(with NEAA)