MEM

Cat. No.: PM150411 Publications(273) Manual
MEM - 1
  • MEMMEM - 1

PM150411

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Product Introduction

Background

MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells.
MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Ingredient Statement
With

• 1000 mg/L D-Glucose

• 2 mM L-Glutamine

• 2200 mg/L NaHCO₃

• 10 mg/L Phenol red

• Earle's Balanced salt

Without

• HEPES

• NEAA

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

Specifications
Concentration
Volume 500mL
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin Content < 3 EU/mL
pH 7.0-7.6
Animal Origin Ingredient Without
Green Features Sustainable packaging
Valid Period 24 months
Storage Condition This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition Room Temperature

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Documents

Publications

Fucoidan alleviates doxorubicin-induced cardiotoxicity by inhibiting ferroptosis via Nrf2/GPX4 pathway

Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024) IF: 7.7

DOI: 10.1016/j.ijbiomac.2024.133792

Product Cited: HL-1 Cells Line, MEM Medium

A separable double-layer self-pumping dressing containing astragaloside for promoting wound healing

Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024) IF: 7.7

DOI: 10.1016/j.ijbiomac.2024.136342

Product Cited: MEM Medium, Calcein-AM/PI Double Staining Kit, Phosphate Buffer (PBS, 1 ×)

The integration of multidisciplinary approaches revealed PTGES3 as a novel drug target for breast cancer treatment

Journal: Journal of Translational Medicine (2024) IF: 7.4

DOI: 10.1186/s12967-024-04899-0

Product Cited: MCF 10A Cells Line, MDA-MB-231 Cells Line, MCF7 [MCF-7] Cells Line, DMEM/F12, Donor Equine Serum, DMEM(High glucose), Fetal Bovine Serum, MEM

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