PM150411
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Product Introduction
Background
MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells.
MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
• Earle's Balanced salt
Without
• HEPES
• NEAA
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.0-7.6 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024) IF: 7.7
DOI: 10.1016/j.ijbiomac.2024.133792
Product Cited: HL-1 Cells Line, MEM Medium
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024) IF: 7.7
DOI: 10.1016/j.ijbiomac.2024.136342
Product Cited: MEM Medium, Calcein-AM/PI Double Staining Kit, Phosphate Buffer (PBS, 1 ×)
Journal: Journal of Translational Medicine (2024) IF: 7.4
DOI: 10.1186/s12967-024-04899-0
Product Cited: MCF 10A Cells Line, MDA-MB-231 Cells Line, MCF7 [MCF-7] Cells Line, DMEM/F12, Donor Equine Serum, DMEM(High glucose), Fetal Bovine Serum, MEM
Journal: JOURNAL OF MEDICINAL CHEMISTRY (2024) IF: 7.3
DOI: 10.1021/acs.jmedchem.4c00067
Product Cited: MEM Medium
Journal: Frontiers in Immunology (2024) IF: 7.3
DOI: 10.3389/fimmu.2024.1369116
Product Cited: 100 mM Sodium Pyruvate Solution, 100 ×, MEM Non-Essential Amino Acid Solution (NEAA), 100 ×, MEM Medium, 200 mM L-Alanyl-L-Glutamine Solution, 100 ×