MEM

Cat. No.: PM150411 Publications(273) Manual
MEM - 1
  • MEMMEM - 1

PM150411

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Product Introduction

Background

MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells.
MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Ingredient Statement
With

• 1000 mg/L D-Glucose

• 2 mM L-Glutamine

• 2200 mg/L NaHCO₃

• 10 mg/L Phenol red

• Earle's Balanced salt

Without

• HEPES

• NEAA

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

Specifications
Concentration
Volume 500mL
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin Content < 3 EU/mL
pH 7.0-7.6
Animal Origin Ingredient Without
Green Features Sustainable packaging
Valid Period 24 months
Storage Condition This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition Room Temperature

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Documents

Publications

Cytoprotective effects of C1s enzyme in macrophages in atherosclerosis mediated through the LRP5 and Wnt/β-catenin pathway

Journal: MOLECULAR IMMUNOLOGY (2024) IF: 3.6

DOI: 10.1016/j.molimm.2024.01.002

Product Cited: RAW 264.7 Cells Line, THP-1 Cells Line, H9c2 (2-1) Cells Line, HL-1 Cells Line, Human Aortic Smooth Muscle cells, Human Umbilical Vein Endothelial cells, MEM, DMEM(High glucose), Ham's F-12K, RPMI-1640, Fetal Bovine Serum

Identify CTBP1-DT as an immunological biomarker that promotes lipid synthesis and apoptosis resistance in KIRC

Journal: GENE (2024) IF: 3.5

DOI: 10.1016/j.gene.2024.148403

Product Cited: HK-2 Cells Line, ACHN Cells Line, A-498 Cells Line, 786-O [786-0] Cells Line, OS-RC-2 Cells Line, Caki-1 Cells Line, RPMI-1640, MEM

GOLPH3 inhibits erastin-induced ferroptosis in colorectal cancer cells

Journal: CELL BIOLOGY INTERNATIONAL (2024) IF: 3.3

DOI: 10.1002/cbin.12190

Product Cited: McCoy's 5A Medium, MEM Medium

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