PM150411
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Product Introduction
Background
MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells.
MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
• Earle's Balanced salt
Without
• HEPES
• NEAA
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.0-7.6 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: MOLECULAR IMMUNOLOGY (2024) IF: 3.6
DOI: 10.1016/j.molimm.2024.01.002
Product Cited: RAW 264.7 Cells Line, THP-1 Cells Line, H9c2 (2-1) Cells Line, HL-1 Cells Line, Human Aortic Smooth Muscle cells, Human Umbilical Vein Endothelial cells, MEM, DMEM(High glucose), Ham's F-12K, RPMI-1640, Fetal Bovine Serum
Journal: Frontiers in Oncology (2024) IF: 3.5
DOI: 10.3389/fonc.2024.1338811
Product Cited: Penicillin-Streptomycin Solution, 100 ×, 0.25% Trypsin Solution, RPMI-1640 Medium, MEM Medium
Journal: GENE (2024) IF: 3.5
DOI: 10.1016/j.gene.2024.148403
Product Cited: HK-2 Cells Line, ACHN Cells Line, A-498 Cells Line, 786-O [786-0] Cells Line, OS-RC-2 Cells Line, Caki-1 Cells Line, RPMI-1640, MEM
Journal: CELL BIOLOGY INTERNATIONAL (2024) IF: 3.3
DOI: 10.1002/cbin.12190
Product Cited: McCoy's 5A Medium, MEM Medium
Journal: EXPERIMENTAL CELL RESEARCH (2024) IF: 3.3
DOI: 10.1016/j.yexcr.2024.114341
Product Cited: MEM Medium, MEM (Glucose free) Medium, HK-2 Cells Line