PM150411
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Product Introduction
Background
MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells.
MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
• Earle's Balanced salt
Without
• HEPES
• NEAA
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.0-7.6 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: FOOD AND CHEMICAL TOXICOLOGY (2023) IF: 4.300
DOI: 10.1016/j.fct.2023.113775
Product Cited: MEM, BV2 Cells Line
Journal: CHEMICAL RESEARCH IN TOXICOLOGY (2023) IF: 4.1
DOI: 10.1021/acs.chemrestox.3c00342
Product Cited: MEM
Journal: Cancer Medicine (2023) IF: 4.000
DOI: 10.1002/cam4.5942
Product Cited: U-2 OS [U2OS] Cells Line, MNNG/HOS Cl #5 [R-1059-D] Cells Line, McCoy's 5A, MEM
Journal: Heliyon (2023) IF: 4.0
DOI: 10.1016/j.heliyon.2023.e22593
Product Cited: MEM Medium
Journal: Cancer Medicine (2023) IF: 4.0
DOI: 10.1002/cam4.6594
Product Cited: Phosphate Buffer (PBS, 1 ×), Fetal Bovine Serum, RPMI-1640 Medium, MEM Medium