PM150411
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Product Introduction
Background
MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells.
MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
• Earle's Balanced salt
Without
• HEPES
• NEAA
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.0-7.6 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY (2023) IF: 2.9
Product Cited: MRC-5 Cells Line, MEM, Fetal Bovine Serum, MEM Non-Essential Amino Acid Solution (NEAA), 100×, Penicillin-Streptomycin Solution, 100×
Journal: Chinese Journal of Integrative Medicine (2023) IF: 2.9
DOI: 10.1007/s11655-023-3698-9
Product Cited: Caco-2 Cells Line, HCT 116 Cells Line, HT-29 Cells Line, SW480 [SW-480] Cells Line, MEM, McCoy's 5A, Leibovitz's L-15
Journal: Anti-Cancer Agents in Medicinal Chemistry (2023) IF: 2.800
Journal: HEREDITAS (2023) IF: 2.700
DOI: 10.1186/s41065-023-00275-y
Product Cited: MDA-MB-231 Cells Line, MCF7 [MCF-7] Cells Line, 293T [HEK-293T] Cells Line, MCF 10A Cells Line, Leibovitz's L-15, MEM, DMEM/F12, DMEM(High glucose)
Journal: Journal of Orthopaedic Surgery and Research (2023) IF: 2.6
DOI: 10.1186/s13018-023-04406-z
Product Cited: MG-63 Cells Line, HOS Cells Line, hFOB 1.19 Cells Line, MEM, DMEM(High glucose), 50 mg/mL G-418 Solution