PM150410
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Product Introduction
Background
MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells.
MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
• NEAA
• Earle's Balanced salt
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: Molecular & Cellular Oncology (2024) IF: 2.6
DOI: 10.1080/23723556.2024.2423986
Product Cited: Hela Cells Line, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100 ×, C-33 A Cells Line, MEM, with NEAA Medium
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024) IF: 2.5
DOI: 10.1016/j.bbrc.2024.151142
Product Cited: HK-2 Cells Line, MEM, with NEAA Medium
Journal: MOLECULAR AND CELLULAR PROBES (2024) IF: 2.3
DOI: 10.1016/j.mcp.2024.101988
Product Cited: MG-63 Cells Line, MEM, with NEAA Medium
Journal: KIDNEY & BLOOD PRESSURE RESEARCH (2024) IF: 2.3
DOI: 10.1159/000540483
Product Cited: ACHN Cells Line, MEM, with NEAA Medium, Fetal Bovine Serum, HK-2 Cells Line, 786-O [786-0] Cells Line, OS-RC-2 Cells Line, RPMI-1640 Complete Medium (10)
Journal: MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS (2024) IF: 2.3
DOI: 10.1016/j.mrfmmm.2024.111876
Product Cited: Fetal Bovine Serum, 5637 Cells Line, RPMI-1640 Medium, McCoy's 5A Medium, T24 [T-24] Cells Line, Ham's F-12K Medium, J82 Cells Line, DMEM (High glucose) Medium, MEM, with NEAA Medium, 293T [HEK-293T] Cells Line, Penicillin-Streptomycin Solution, 100 ×, SV-HUC-1 Cells Line, RT4 Cells Line