PM150410
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Product Introduction
Background
MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells.
MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
• NEAA
• Earle's Balanced salt
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
This product is manufactured in a GMP-compliant factory, formulated with water for injection, and has obtained ISO 9001 and ISO 13485 certifications.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Medium | Components 1000 mg/L D-Glucose 2 mM L-Glutamine 2200 mg/L NaHCO₃ NEAA Earle's 10 mg/L Phenol Red |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Publications
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH (2023) IF: 5.1
DOI: 10.1016/j.bbamcr.2023.119509
Product Cited: A172 Cells Line, U-87 MG Cells Line, U-118 MG Cells Line, U251 Cells Line, T98G Cells Line, DMEM(High glucose), Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×, MEM(with NEAA)
Journal: Translational Oncology (2023) IF: 5.0
DOI: 10.1016/j.tranon.2023.101839
Product Cited: 5 mg/mL MTT solution, MEM Medium, Hela Cells Line
Journal: JOURNAL OF BIOLOGICAL CHEMISTRY (2023) IF: 4.800
DOI: 10.1016/j.jbc.2023.104621
Product Cited: McCoy's 5A, MEM(with NEAA), RPMI-1640, DMEM(High glucose), Renca [RenCa] Cells Complete Medium
Journal: CARCINOGENESIS (2023) IF: 4.7
Product Cited: HCT 116 Cells Line, LS180 Cells Line, McCoy's 5A, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×, MEM(with NEAA)
Journal: MOLECULES (2023) IF: 4.6
DOI: 10.3390/molecules28166128
Product Cited: MEM(with NEAA)
