PM150422
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Product Introduction
Background
MEMα was developed on the basis of MEM medium. Compared with MEM medium, NEAA, sodium pyruvate, zinc sulfate, VB12, biotin and ascorbic acid were added. It is widely used in suspension and adherent cell culture of various mammals. MEMα medium without nucleoside and deoxynucleoside is often used as medium for DG44 and other DHFR-cells.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
• 1 mM Sodium pyruvate
• Earle's Balanced salt
Without
• HEPES
• Nucleoside
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.0-7.6 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: IMMUNOLOGY (2024) IF: 6.4
DOI: 10.1111/imm.13768
Product Cited: Penicillin-Streptomycin Solution, 100×, NK-92 Cells Line, MEMα, without nucleoside, Recombinant Human Interleukin-2 (IL-2) Solution, Donor Equine Serum, Fetal Bovine Serum
Journal: Advanced Science (2024) IF: 14.3
Product Cited: Donor Equine Serum, MEMα, without nucleoside Medium, Fetal Bovine Serum, NK-92 Cells Line
Journal: Science China-Materials (2022) IF: 8.600
DOI: 10.1007/s40843-022-2205-1
Product Cited: NK-92 Cells Line, MEMα, without nucleoside, β-Mercaptoethanol, Donor Equine Serum, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×