Mergene1000^® Hela Cell-Specific mRNA Transfection Reagent

Product Introduction

Background

Mergene1000^® Hela Cell-Specific mRNA Transfection Reagent is a high-performance mRNA transfection reagent designed for the delivery of mRNA. It can directly deliver mRNA into the cytoplasm for expression, thereby avoiding the limitations of transcriptional regulation and entry into the nucleus. It is specifically formulated for use with Hela cells, achieving high transfection efficiency. The reagent is distinguished by its low toxicity, excellent stability, ease of operation, and high reproducibility.

Matters Need Attention

1. The cell inoculation amount and transfection ratio provided above are based on experiments conducted with Hela cells and are for reference only. The specific experimental dosage should be adjusted according to the actual conditions.
2. The product is transported with ice bag and can be aliquoted and stored upon use to avoid multiple prolonged openings of the lid.
3. MEM, with NEAA medium should be prepared separately for the dilution of mRNA and transfection reagents.
4. During transfection, ensure that the degree of cell confluence is not less than 70%, and it is generally maintained at around 70% to 90%. The specific plating density can be adjusted according to the actual conditions of the cells.
5. After transfection, there is no need to remove the transfection complex or replace with fresh culture medium. The actual operation can be based on the cell status, after transfection culture 4-6 hours to choose to change the medium.
6. The use of high purity mRNA is helpful to obtain higher transfection efficiency.
7. The experimental process utilized RNA-free and pyrogen-free materials, such as centrifuge tubes, pipette tips, and buffers.
8. For research use only.
9. For your safety and health, please wear experimental clothes and wear disposable gloves aseptic operation.