PB180120
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Product Introduction
Background
Penicillin-streptomycin solution mixture is the most commonly used antibiotic to prevent microbial contamination in vitro. Penicillin can interfere with the synthesis of bacterial cell wall, especially for Gram-positive bacteria. Streptomycin could bind to ribosomal ribosome for 30S and inhibit the synthesis of bacterial protein. It was effective for Gram-negative bacteria and Gram-positive bacteria, but especially for Gram-negative bacteria. The combined use of penicillin and streptomycin can prevent most bacterial contamination, but penicillin solution is sensitive to temperature and pH, easy to degrade at room temperature, and needs cryopreservation, the stability of penicillin solution is the most stable when pH is 6.0-6.5. Streptomycin was relatively stable, and pH 5.0-7.5 was the most stable.
Ingredient Statement
With
• 10 mM PBS (pH 6.2) Dissolvent
• 10 mg/mL Streptomycin sulfate
• 10 kU/mL Penicillin G
• Gram-positive bacteria & Gram-negative bacteria Antimicrobial spectrum
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the pollution.
4. It is not suitable for long time storage at room temperature or 2-8℃.
5. Thaw in temperature or 37℃ and mix fully before use. Avoid repeated freezing and thawing. It is recommended to aliquot the solution and store at -5~-20℃.
6. This product is a concentrated solution and dilute it to the desired concentration before use.
Specifications
Concentration | 100× |
Volume | 125mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 6.0-6.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 12 months |
Storage Condition | This product can be stored at -5~-20℃ for 12 months with shading light. |
Shipping Condition | Ice bag |
Publications
Journal: HISTOLOGY AND HISTOPATHOLOGY (2022) IF: 2.130
DOI: 10.14670/HH-18-467
Product Cited: MEM, with NEAA Medium, Penicillin-Streptomycin Solution, 100 ×, Fetal Bovine Serum, HUV-EC-C [HUVEC] Cells Line, Ham's F-12K Medium, RPMI-1640 Medium
Journal: Reproductive Biology (2022) IF: 2.100
DOI: 10.1016/j.repbio.2022.100704
Product Cited: SK-OV-3 Cells Line, NIH:OVCAR-3 [OVCAR3] Cells Line, A2780 Cells Line, ES-2 Cells Line, 293T [HEK-293T] Cells Line, RPMI-1640, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×
Journal: BIOMATERIALS (2022) IF: 15.304
DOI: 10.1016/j.biomaterials.2022.121739
Product Cited: Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100 ×, MEM (ATCC modified) Medium
Journal: ENVIRONMENT INTERNATIONAL (2022) IF: 13.400
DOI: 10.1016/j.envint.2022.107568
Product Cited: 4T1 Cells Line, RPMI-1640, Penicillin-Streptomycin Solution, 100×, RPMI-1640, without phenol red
Journal: Autophagy (2022) IF: 13.391
DOI: 10.1080/15548627.2022.2084686
Product Cited: Penicillin-Streptomycin Solution, 100 ×, Phosphate Buffer (PBS, 1 ×)