PB180120
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Product Introduction
Background
Penicillin-streptomycin solution mixture is the most commonly used antibiotic to prevent microbial contamination in vitro. Penicillin can interfere with the synthesis of bacterial cell wall, especially for Gram-positive bacteria. Streptomycin could bind to ribosomal ribosome for 30S and inhibit the synthesis of bacterial protein. It was effective for Gram-negative bacteria and Gram-positive bacteria, but especially for Gram-negative bacteria. The combined use of penicillin and streptomycin can prevent most bacterial contamination, but penicillin solution is sensitive to temperature and pH, easy to degrade at room temperature, and needs cryopreservation, the stability of penicillin solution is the most stable when pH is 6.0-6.5. Streptomycin was relatively stable, and pH 5.0-7.5 was the most stable.
Ingredient Statement
With
• 10 mM PBS (pH 6.2) Dissolvent
• 10 mg/mL Streptomycin sulfate
• 10 kU/mL Penicillin G
• Gram-positive bacteria & Gram-negative bacteria Antimicrobial spectrum
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the pollution.
4. It is not suitable for long time storage at room temperature or 2-8℃.
5. Thaw in temperature or 37℃ and mix fully before use. Avoid repeated freezing and thawing. It is recommended to aliquot the solution and store at -5~-20℃.
6. This product is a concentrated solution and dilute it to the desired concentration before use.
Specifications
Concentration | 100× |
Volume | 125mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 6.0-6.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 12 months |
Storage Condition | This product can be stored at -5~-20℃ for 12 months with shading light. |
Shipping Condition | Ice bag |
Publications
Journal: Phytomedicine (2021) IF: 7.900
DOI: 10.1016/j.phymed.2021.153614
Product Cited: Penicillin-Streptomycin Solution, 100×
Journal: Analytical Chemistry (2021) IF: 7.400
DOI: 10.1021/acs.analchem.1c02596
Product Cited: Penicillin-Streptomycin Solution, 100×
Journal: International Journal Of Pharmaceutics (2021) IF: 5.800
DOI: 10.1016/j.ijpharm.2021.121083
Product Cited: 0.25% Trypsin Solution, Penicillin-Streptomycin Solution, 100×, Phosphate Buffer (PBS)
Journal: Cancer Cell International (2021) IF: 5.800
DOI: 10.1186/s12935-021-02290-3
Product Cited: SW620 Cells Line, LoVo Cells Line, HCT 116 Cells Line, SW480 [SW-480] Cells Line, RPMI-1640, DMEM(High glucose), Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×
Journal: Cancer Cell International (2021) IF: 5.800
DOI: 10.1186/s12935-021-02293-0
Product Cited: SHG-44 Cells Line, A172 Cells Line, U251 Cells Line, PaTu 8988t Cells Line, DMEM(High glucose), Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×