PB180120
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Product Introduction
Background
Penicillin-streptomycin solution mixture is the most commonly used antibiotic to prevent microbial contamination in vitro. Penicillin can interfere with the synthesis of bacterial cell wall, especially for Gram-positive bacteria. Streptomycin could bind to ribosomal ribosome for 30S and inhibit the synthesis of bacterial protein. It was effective for Gram-negative bacteria and Gram-positive bacteria, but especially for Gram-negative bacteria. The combined use of penicillin and streptomycin can prevent most bacterial contamination, but penicillin solution is sensitive to temperature and pH, easy to degrade at room temperature, and needs cryopreservation, the stability of penicillin solution is the most stable when pH is 6.0-6.5. Streptomycin was relatively stable, and pH 5.0-7.5 was the most stable.
Ingredient Statement
With
• 10 mM PBS (pH 6.2) Dissolvent
• 10 mg/mL Streptomycin sulfate
• 10 kU/mL Penicillin G
• Gram-positive bacteria & Gram-negative bacteria Antimicrobial spectrum
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the pollution.
4. It is not suitable for long time storage at room temperature or 2-8℃.
5. Thaw in temperature or 37℃ and mix fully before use. Avoid repeated freezing and thawing. It is recommended to aliquot the solution and store at -5~-20℃.
6. This product is a concentrated solution and dilute it to the desired concentration before use.
Specifications
Concentration | 100× |
Volume | 125mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 6.0-6.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 12 months |
Storage Condition | This product can be stored at -5~-20℃ for 12 months with shading light. |
Shipping Condition | Ice bag |
Publications
Journal: PHYTOMEDICINE (2024) IF: 7.9
DOI: 10.1016/j.phymed.2024.155537
Product Cited: Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×, RPMI-1640
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024) IF: 7.7
DOI: 10.1016/j.ijbiomac.2024.135736
Product Cited: Penicillin-Streptomycin Solution, 100 ×, RAW 264.7 Cells Line
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024) IF: 7.7
DOI: 10.1016/j.ijbiomac.2024.137460
Product Cited: Ham's F-12K Medium, Penicillin-Streptomycin Solution, 100 ×, Fetal Bovine Serum
Journal: Journal of Environmental Chemical Engineering (2024) IF: 7.7
DOI: 10.1016/j.jece.2024.112841
Product Cited: Penicillin-Streptomycin Solution, 100 ×
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024) IF: 7.7
DOI: 10.1016/j.ijbiomac.2024.134787
Product Cited: Phosphate Buffer (PBS, 1 ×), DMEM (High glucose) Medium, Hep G2 Cells Line, HL-7702 [L-02; LO2] Cells Line, RPMI-1640 Medium, 0.25% Trypsin Solution, Penicillin-Streptomycin Solution, 100 ×, Fetal Bovine Serum