Poly-L-Lysine Solution (1 × )

Product Introduction

Background

This product is used for a part of the cells in the culture process without the use of coating reagents to promote adherence, which will affect the normal growth of cells. It is only used for cell culture.

Matters Need Attention

1. The amount of coating solution depends on different culture vessels. Generally, it is ensured that the bottom of the culture vessel is completely covered, and the coating should not be dried within the time.
2. Poly-L-lysine is mildly toxic. Therefore, the coated culture vessel must be cleaned 3 times with PBS or medium before use.
3. The coating process includes two stages: coating time and drying time. Coating time is the period during which the coating solution adheres to the surface

it is advisable to follow the recommended time since longer durations do not improve the effect.The drying time is the period allowed for the coated culture vessels to air dry after coating, ensuring that the coating solution is completely dry and achieves optimal adhesive properties.
4. The effect of the coated liquid is not affected when it is transported at normal temperature for 3-5 days. However, it should be stored according to storage conditions.
5. All the above operations are based on sterile reagents, consumables, and operation in a sterile environment.