PM150110
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Product Introduction
Background
RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media.
RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.
Ingredient Statement
With
• 2000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2000 mg/L NaHCO₃
• 5 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
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Publications
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY (2025) IF: 4.8
DOI: 10.1016/j.intimp.2025.114015
Product Cited: DMEM/F12 Medium, RPMI-1640 Medium, DMEM (High glucose) Medium
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY (2025) IF: 4.7
DOI: 10.1016/j.intimp.2025.115072
Product Cited: RPMI-1640 Medium, HT-29 Cells Line, HCT 116 Cells Line
Journal: NEUROPHARMACOLOGY (2025) IF: 4.6
DOI: 10.1016/j.neuropharm.2025.110405
Product Cited: RPMI-1640 Medium, Penicillin-Streptomycin Solution, 100 ×, PC-12 (High differentiation) Cells Line, Fetal Bovine Serum
Journal: iScience (2025) IF: 4.6
DOI: 10.1016/j.isci.2025.112526
Product Cited: Donor Equine Serum, BT-549 Cells Line, DMEM/F12 Medium, MCF 10A Cells Line, MDA-MB-231 Cells Line, Penicillin-Streptomycin Solution, 100 ×, RPMI-1640 Medium, 10 mg/mL Recombinant Human Insulin Solution, MEM Non-Essential Amino Acid Solution (NEAA), 100 ×, HCC1937 Cells Line, MDA-MB-468-06 Cells Line, Leibovitz's L-15 Medium, EGF/Urogastrone/URG, Human, Recombinant, Fetal Bovine Serum
Journal: iScience (2025) IF: 4.6
DOI: 10.1016/j.isci.2025.112379
Product Cited: RPMI-1640 Medium
