RPMI-1640

Cat. No.: PM150110 Publications(915) Manual
RPMI-1640 - 1
  • RPMI-1640RPMI-1640 - 1

PM150110

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500mL

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Product Introduction

Background

RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media.
RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

Ingredient Statement
With

• 2000 mg/L D-Glucose

• 2 mM L-Glutamine

• 2000 mg/L NaHCO₃

• 5 mg/L Phenol red

Without

• HEPES

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

System Certification

GMP Production and Quality System
This product is manufactured in a GMP-compliant factory, formulated with water for injection, and has obtained ISO 9001 and ISO 13485 certifications.

Specifications
Concentration
Volume 500mL
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin Content < 3 EU/mL
pH 7.2-7.4
Medium Components 2000 mg/L D-Glucose
2 mM L-Glutamine
2000 mg/L NaHCO₃
5 mg/L Phenol Red
Animal Origin Ingredient Without
Green Features Sustainable packaging
Valid Period 24 months
Storage Condition This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition Room Temperature

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Documents

Publications

Tim-3 Expression Causes NK Cell Dysfunction in Type 2 Diabetes Patients

Journal: Frontiers in Immunology (2022) IF: 8.800

DOI: 10.3389/fimmu.2022.852436

Product Cited: RPMI-1640

Overcoming the resistance of hepatocellular carcinoma to PD-1/PD-L1 inhibitor and the resultant immunosuppression by CD38 siRNA-loaded extracellular vesicles

Journal: OncoImmunology (2022) IF: 7.700

DOI: 10.1080/2162402X.2022.2152635

Product Cited: HuH-7 Cells Line, HCCLM3 Cells Line, MHCC97-L Cells Line, RPMI-1640, BNL 1ME A.7R.1 Cells Line, DMEM(High glucose), Mouse Bone Marrow Mesenchymal Stem cells, Hank's Balanced Salt Solution (HBSS), with calcium, magnesium, without phenol red

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