RPMI-1640

Cat. No.: PM150110 Publications(915) Manual
RPMI-1640 - 1
  • RPMI-1640RPMI-1640 - 1

PM150110

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500mL

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Product Introduction

Background

RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media.
RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

Ingredient Statement
With

• 2000 mg/L D-Glucose

• 2 mM L-Glutamine

• 2000 mg/L NaHCO₃

• 5 mg/L Phenol red

Without

• HEPES

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

Specifications
Concentration
Volume 500mL
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin Content < 3 EU/mL
pH 7.2-7.4
Animal Origin Ingredient Without
Green Features Sustainable packaging
Valid Period 24 months
Storage Condition This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition Room Temperature

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Documents

Publications

BMP6 ubiquitination mediated by SMURF1 suppresses ferroptosis and diminishes sensitivity to doxorubicin in gastric cancer

Journal: Gastroenterology Report (2025) IF: 4.2

DOI: 10.1093/gastro/goaf051

Product Cited: RPMI-1640 Medium, HGC-27 Cells Line, AGS Cells Line, MKN-45 Cells Line

Oncogenic HNF4α inhibits ferroptotic cell death through activating SLC7A11 by recruiting p300/CBP in breast cancer

Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2025) IF: 4.2

DOI: 10.1016/j.bbadis.2025.167884

Product Cited: DMEM (High glucose) Medium, SUM159PT Cells Line, RPMI-1640 Medium, MDA-MB-231 Cells Line, 293T [HEK-293T] Cells Line

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