PM150110
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Product Introduction
Background
RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media.
RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.
Ingredient Statement
With
• 2000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2000 mg/L NaHCO₃
• 5 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
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Publications
Journal: MOLECULAR AND CELLULAR BIOCHEMISTRY (2025) IF: 3.5
DOI: 10.1007/s11010-025-05267-2
Product Cited: Capan-2 Cells Line, SW 1990 Cells Line, CFPAC-1 Cells Line, RPMI-1640 Medium, DMEM (High glucose) Medium, PANC-1 Cells Line, Capan-1 Cells Line, BxPC-3 Cells Line
Journal: Frontiers in Oncology (2025) IF: 3.5
DOI: 10.3389/fonc.2024.1536406
Product Cited: 4T1 Cells Line, Penicillin-Streptomycin Solution, 100 ×, Fetal Bovine Serum, RPMI-1640 Medium, Phosphate Buffer (PBS, 1 ×), MDA-MB-231 Cells Line, DMEM (High glucose) Medium
Journal: Human Cell (2025) IF: 3.4
DOI: 10.1007/s13577-025-01185-z
Product Cited: JEG-3 Cells Line, RPMI-1640 Medium, Penicillin-Streptomycin Solution, 100 ×, HTR-8-Svneo Cells Line
Journal: BMC CANCER (2025) IF: 3.4
DOI: 10.1186/s12885-025-13973-9
Product Cited: RPMI-1640 Medium, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100 ×
Journal: Molecular Medicine Reports (2025) IF: 3.4
Product Cited: RPMI-1640 Medium
