PM150110
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Product Introduction
Background
RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media.
RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.
Ingredient Statement
With
• 2000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2000 mg/L NaHCO₃
• 5 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
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Publications
Journal: Journal of Cancer (2025) IF: 3.3
DOI: 10.7150/jca.100621
Product Cited: DMEM (High glucose) Medium, GES-1 Cells Line, RPMI-1640 Medium, 293 [HEK-293] Cells Line
Journal: BIOORGANIC & MEDICINAL CHEMISTRY (2025) IF: 3.3
DOI: 10.1016/j.bmc.2025.118087
Product Cited: Penicillin-Streptomycin Solution, 100 ×, Fetal Bovine Serum, RPMI-1640 Medium
Journal: CELL BIOLOGY INTERNATIONAL (2025) IF: 3.3
DOI: 10.1002/cbin.12274
Product Cited: TPC-1 Cells Line, Nthy-ori 3-1 Cells Line, RPMI-1640 Medium, FTC-133 Cells Line
Journal: Advanced Biology (2025) IF: 3.2
Product Cited: SK-OV-3 Cells Line, Penicillin-Streptomycin Solution, 100 ×, Human Oral Epithelial Cells, Human Ovarian Epithelial Cell Complete Medium, NIH:OVCAR-3 [OVCAR3] Cells Line, 10 mg/mL Recombinant Human Insulin Solution, McCoy's 5A Medium, RPMI-1640 Medium, Caov-3 Cells Line
Journal: JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2025) IF: 3.2
DOI: 10.1002/jbt.70303
Product Cited: Fetal Bovine Serum, RPMI-1640 Medium, Penicillin-Streptomycin Solution, 100 ×, HT22 Cells Line, DMEM (High glucose) Medium
