PM150110
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Product Introduction
Background
RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media.
RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.
Ingredient Statement
With
• 2000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2000 mg/L NaHCO₃
• 5 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
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Publications
Journal: Frontiers in Medicine (2025) IF: 3.1
DOI: 10.3389/fmed.2025.1503472
Product Cited: Fetal Bovine Serum, DMEM (High glucose) Medium, RPMI-1640 Medium, MEM Medium, Penicillin-Streptomycin Solution, 100 ×, RH-35 Cells Line, HFF-1 Cells Line, 0.25% Trypsin Solution
Journal: RENAL FAILURE (2025) IF: 3.0
DOI: 10.1080/0886022X.2024.2438859
Product Cited: Interferon γ/IFN-γ/IFNG, Human, Recombinant, Human Renal Podocyte Cells, RPMI-1640 Medium
Journal: MOLECULAR IMMUNOLOGY (2025) IF: 3
DOI: 10.1016/j.molimm.2025.06.004
Product Cited: THP-1 Cells Line, RPMI-1640 Medium, Caco-2 Cells Line, MEM Medium
Journal: MOLECULAR CARCINOGENESIS (2025) IF: 3
DOI: 10.1002/mc.23926
Product Cited: RPMI-1640 Medium
Journal: ADVANCED MATERIALS (2025) IF: 27.4
Product Cited: RPMI-1640 Medium, PC-12 (Undifferentiated) Cells Line, Donor Equine Serum
