PM150110
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Product Introduction
Background
RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media.
RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.
Ingredient Statement
With
• 2000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2000 mg/L NaHCO₃
• 5 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
This product is manufactured in a GMP-compliant factory, formulated with water for injection, and has obtained ISO 9001 and ISO 13485 certifications.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Medium | Components 2000 mg/L D-Glucose 2 mM L-Glutamine 2000 mg/L NaHCO₃ 5 mg/L Phenol Red |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: GENE (2025) IF: 2.6
DOI: 10.1016/j.gene.2025.149313
Product Cited: MEM Medium, RPMI-1640 Medium, McCoy's 5A Medium, DMEM (High glucose) Medium
Journal: BMC Pulmonary Medicine (2025) IF: 2.6
DOI: 10.1186/s12890-025-03631-y
Product Cited: DMEM (High glucose) Medium, Penicillin-Streptomycin Solution, 100 ×, RPMI-1640 Medium, NCI-H1650 Cells Line
Journal: TOXICON (2025) IF: 2.6
DOI: 10.1016/j.toxicon.2025.108366
Product Cited: Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100 ×, RPMI-1640 Medium, Phosphate Buffer (PBS, 1 ×)
Journal: BMC Pulmonary Medicine (2025) IF: 2.6
DOI: 10.1186/s12890-025-03620-1
Product Cited: NCI-H1975 Cells Line, DMEM (High glucose) Medium, RPMI-1640 Medium, Fetal Bovine Serum, NCI-H441 Cells Line
Journal: Endocrine Connections (2025) IF: 2.6
DOI: 10.1530/EC-25-0022
Product Cited: Fetal Bovine Serum, RPMI-1640 Medium