RPMI-1640

Cat. No.: PM150110 Publications(915) Manual
RPMI-1640 - 1
  • RPMI-1640RPMI-1640 - 1

PM150110

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500mL

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Product Introduction

Background

RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media.
RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

Ingredient Statement
With

• 2000 mg/L D-Glucose

• 2 mM L-Glutamine

• 2000 mg/L NaHCO₃

• 5 mg/L Phenol red

Without

• HEPES

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

System Certification

GMP Production and Quality System
This product is manufactured in a GMP-compliant factory, formulated with water for injection, and has obtained ISO 9001 and ISO 13485 certifications.

Specifications
Concentration
Volume 500mL
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin Content < 3 EU/mL
pH 7.2-7.4
Medium Components 2000 mg/L D-Glucose
2 mM L-Glutamine
2000 mg/L NaHCO₃
5 mg/L Phenol Red
Animal Origin Ingredient Without
Green Features Sustainable packaging
Valid Period 24 months
Storage Condition This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition Room Temperature

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Documents

Publications

HMGA1 sensitizes esophageal squamous cell carcinoma to mTOR inhibitors through the ETS1-FKBP12 axis

Journal: International Journal of Biological Sciences (2024) IF: 9.2

DOI: 10.7150/ijbs.95595

Product Cited: RPMI-1640 Medium

N6-methyladenosine-modified SRPK1 promotes aerobic glycolysis of lung adenocarcinoma via PKM splicing

Journal: CELLULAR & MOLECULAR BIOLOGY LETTERS (2024) IF: 9.2

DOI: 10.1186/s11658-024-00622-5

Product Cited: NCI-H1975 Cells Line, NCI-H1650 Cells Line, 293T [HEK-293T] Cells Line, DMEM (High glucose) Medium, PC-9 Cells Line, A549 [A-549] Cells Line, RPMI-1640 Medium, NCI-H1299 Cells Line, HCC827 Cells Line, NCI-H226 [H226] Cells Line, BEAS-2B Cells Line

TRIM65 promotes renal cell carcinoma through ubiquitination and degradation of BTG3

Journal: Cell Death & Disease (2024) IF: 9.0

DOI: 10.1038/s41419-024-06741-3

Product Cited: 769-P Cells Line, Caki-1 Cells Line, RPMI-1640 Medium, Caki-2 Cells Line, ACHN Cells Line, MEM Medium, McCoy's 5A Medium, DMEM (High glucose) Medium

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