PM150110
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Product Introduction
Background
RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media.
RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.
Ingredient Statement
With
• 2000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2000 mg/L NaHCO₃
• 5 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: PHYTOMEDICINE (2025) IF: 6.7
DOI: 10.1016/j.phymed.2025.156645
Product Cited: DMEM (High glucose) Medium, RPMI-1640 Medium
Journal: ANALYTICAL CHEMISTRY (2025) IF: 6.7
DOI: 10.1021/acs.analchem.5c00365
Product Cited: RPMI-1640 Medium
Journal: International Journal of Nanomedicine (2025) IF: 6.6
DOI: 10.2147/IJN.S510964
Product Cited: RPMI-1640 Medium
Journal: EMBO REPORTS (2025) IF: 6.5
DOI: 10.1038/s44319-025-00385-w
Product Cited: RPMI-1640 Medium, SK-BR-3 [SKBR3] Cells Line, T-47D Cells Line, HCC1937 Cells Line, BT-549 Cells Line, ZR-75-1 Cells Line, JeKo-1 Cells Line, 293FT Cells Line, MDA-MB-436 Cells Line, Jurkat, Clone E6-1 Cells Line, McCoy's 5A Medium, DMEM (High glucose) Medium, BT-474 [BT474] Cells Line
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2025) IF: 6.2
DOI: 10.1016/j.ecoenv.2025.117728
Product Cited: Fetal Bovine Serum, RPMI-1640 Medium, HTR-8-Svneo Cells Line