PM150110
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Product Introduction
Background
RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media.
RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.
Ingredient Statement
With
• 2000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2000 mg/L NaHCO₃
• 5 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
This product is manufactured in a GMP-compliant factory, formulated with water for injection, and has obtained ISO 9001 and ISO 13485 certifications.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Medium | Components 2000 mg/L D-Glucose 2 mM L-Glutamine 2000 mg/L NaHCO₃ 5 mg/L Phenol Red |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: JCI Insight (2024) IF: 6.3
DOI: 10.1172/jci.insight.178535
Product Cited: DMEM (High glucose) Medium, RPMI-1640 Medium, MEM Medium
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2024) IF: 6.2
DOI: 10.1016/j.ecoenv.2024.117331
Product Cited: β-Mercaptoethanol, 55 mM (1000 × ), DMEM (High glucose) Medium, Penicillin-Streptomycin Solution, 100 ×, RPMI-1640 Medium, Fetal Bovine Serum
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2024) IF: 6.2
DOI: 10.1016/j.bbadis.2024.167050
Product Cited: DMEM/F12, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×, MEM, RPMI-1640
Journal: Journal of Translational Medicine (2024) IF: 6.1
DOI: 10.1186/s12967-024-05398-y
Product Cited: RPMI-1640 Medium, THP-1 Cells Line
Journal: Journal of Translational Medicine (2024) IF: 6.1
DOI: 10.1186/s12967-024-05803-6
Product Cited: RPMI-1640 Medium