William's E, with L-glutamine

Cat. No.: PM151221 Publications(2) Manual

PM151221

$ 40.00

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500mL

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Product Introduction Related Products Documents Publications

Product Introduction

Background

William's E medium was designed by William and Gunn. It is mainly used for the long-term culture of rat liver epithelial cells, and can also be used for the culture of other mammalian liver cells. L-glutamine is a necessary nutrient element in cell culture, but it is unstable in solution and will spontaneously degrade. The medium without L-glutamine can add fresh L-glutamine or its substitute arbitrarily according to research needs.
William's medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Ingredient Statement

With

• 2000 mg/L D-Glucose

• 2 mM L-Glutamine

• 2200 mg/L NaHCO₃

• 10 mg/L Phenol red

• 25 mg/L Sodium Pyruvate

• 0.05 mg/L Glutathione

• 0.03 mg/L Methyl Linoleate

Without

• HEPES

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

Specifications

Concentration	1×
Volume	500mL
Form	Liquid
Bacteria Detection	Negative
Fungi Detection	Negative
Mycoplasma Detection	Negative
Endotoxin Content	< 3 EU/mL
pH	7.2-7.4
Animal Origin Ingredient	Without
Green Features	Sustainable packaging
Valid Period	24 months
Storage Condition	This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition	Room Temperature