PM150312
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Product Introduction
Background
The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3151 mg/L D-Glucose
• 2.5 mM L-Glutamine
• 2438 mg/L NaHCO₃
• 8.1 mg/L Phenol red
• 0.5 mM Sodium pyruvate
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
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Publications
Journal: Open Medicine (2023) IF: 2.100
Product Cited: MCF 10A Cells Line, MCF7 [MCF-7] Cells Line, MDA-MB-231 Cells Line, MDA-MB-436 Cells Line, SK-BR-3 [SKBR3] Cells Line, DMEM/F12, Donor Equine Serum, MEM Non-Essential Amino Acid Solution (NEAA), 100×, Penicillin-Streptomycin Solution, 100×, RPMI-1640 (ATCC Modification), Leibovitz's L-15, McCoy's 5A, Fetal Bovine Serum
Journal: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2023) IF: 2.1
DOI: 10.1007/s11626-023-00826-7
Product Cited: DMEM/F12
Journal: Reproductive Biology (2023) IF: 2.1
DOI: 10.1016/j.repbio.2023.100782
Product Cited: Rat Ovarian Granulosa cells, DMEM/F12, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×
Journal: Translational Andrology and Urology (2023) IF: 2.000
DOI: 10.21037/tau-22-872
Product Cited: DMEM/F12 Medium, Leibovitz's L-15 Medium, RPMI-1640 Medium, McCoy's 5A Medium
Journal: HISTOLOGY AND HISTOPATHOLOGY (2023) IF: 2.000
DOI: 10.14670/HH-18-624
Product Cited: 0.25% Trypsin Solution, DMEM/F12, Rat Astrocyte cells Complete Medium
