PM150140
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Product Introduction
Background
RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. The ATCC-modified RPMI-1640 adds sodium pyruvate and HEPES to increase the sugar content and decrease the Sodium bicarbonate content.
RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.
Ingredient Statement
With
• 4500 mg/L D-Glucose
• 10 mM HEPES
• 2 mM L-Glutamine
• 1500 mg/L NaHCO₃
• 5 mg/L Phenol red
• 1 mM Sodium Pyruvate
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: Neural Regeneration Research (2024) IF: 6.1
DOI: 10.4103/NRR.NRR-D-23-01355
Product Cited: THP-1 Cells Line, RPMI-1640 (ATCC Modification), Penicillin-Streptomycin Solution, 100×, β-Mercaptoethanol
Journal: Pharmaceuticals (2024) IF: 4.3
DOI: 10.3390/ph17081088
Product Cited: DMEM (High glucose) Medium, RPMI-1640 Medium (ATCC Modification)
Journal: Chemical Biology & Drug Design (2024) IF: 3.0
DOI: 10.1111/cbdd.14429
Product Cited: MCF 10A Cells Line, MCF7 [MCF-7] Cells Line, MDA-MB-231 Cells Line, RPMI-1640 (ATCC Modification), Leibovitz's L-15
Journal: Open Medicine (2023) IF: 2.100
Product Cited: MCF 10A Cells Line, MCF7 [MCF-7] Cells Line, MDA-MB-231 Cells Line, MDA-MB-436 Cells Line, SK-BR-3 [SKBR3] Cells Line, DMEM/F12, Donor Equine Serum, MEM Non-Essential Amino Acid Solution (NEAA), 100×, Penicillin-Streptomycin Solution, 100×, RPMI-1640 (ATCC Modification), Leibovitz's L-15, McCoy's 5A, Fetal Bovine Serum