PM150312
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Product Introduction
Background
The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3151 mg/L D-Glucose
• 2.5 mM L-Glutamine
• 2438 mg/L NaHCO₃
• 8.1 mg/L Phenol red
• 0.5 mM Sodium pyruvate
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
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Publications
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2025) IF: 4.2
DOI: 10.1016/j.bbadis.2025.167815
Product Cited: Insulin-Transferrin-Selenium (ITS-G), 100 ×, DMEM/F12 Medium, Fetal Bovine Serum
Journal: Journal of Cell Communication and Signaling (2025) IF: 3.9
DOI: 10.1002/ccs3.70024
Product Cited: DMEM/F12 Medium, 293T [HEK-293T] Cells Line
Journal: FOOD AND CHEMICAL TOXICOLOGY (2025) IF: 3.9
DOI: 10.1016/j.fct.2025.115574
Product Cited: EGF/Urogastrone/URG, Human, Recombinant, Donor Equine Serum, MEM Non-Essential Amino Acid Solution (NEAA), 100 ×, Penicillin-Streptomycin Solution, 100 ×, DMEM/F12 Medium, 10 mg/mL Recombinant Human Insulin Solution
Journal: MOLECULAR DIVERSITY (2025) IF: 3.9
DOI: 10.1007/s11030-025-11185-8
Product Cited: HK-2 Cells Line, DMEM/F12 Medium, U251 Cells Line, U-87 MG Cells Line, 0.05% Trypsin Solution, with EDTA, dissolved in PBS, Annexin V-FITC/PI Apoptosis Kit, DMEM (High glucose) Medium, Penicillin-Streptomycin Solution, 100 ×, A172 Cells Line
Journal: Journal of Ovarian Research (2025) IF: 3.8
DOI: 10.1186/s13048-025-01641-2
Product Cited: RPMI-1640 Medium, Penicillin-Streptomycin Solution, 100 ×, DMEM/F12 Medium
