PM150210
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Product Introduction
Background
DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times.
The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucose type), which has been widely used in cell culture.
DMEM (High glucose) was widely used in fast growth, low adhesion cells, hybridoma myeloma cells, clone cells, DNA transfected transformation cells, various primary virus host cells, single cell culture and vaccine production.
DMEM (High glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 4500 mg/L D-Glucose
• 4 mM L-Glutamine
• 3700 mg/L NaHCO₃
• 15 mg/L Phenol red
• 1 mM Sodium pyruvate
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: SCIENCE OF THE TOTAL ENVIRONMENT (2022) IF: 10.800
DOI: 10.1016/j.scitotenv.2022.158813
Product Cited: DMEM(High glucose)
Journal: CARBOHYDRATE POLYMERS (2022) IF: 10.723
DOI: 10.1016/j.carbpol.2022.120233
Product Cited: MEM, with NEAA Medium, DMEM (High glucose) Medium
Journal: PHARMACOLOGICAL RESEARCH (2022) IF: 10.300
DOI: 10.1016/j.phrs.2022.106606
Product Cited: DMEM(High glucose), DMEM/F12
Journal: NEUROREPORT (2022) IF: 1.700
DOI: 10.1097/WNR.0000000000001848
Product Cited: HT22 Cells Line, HT22 Cells Complete Medium, DMEM(High glucose), Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×, DMEM(Glucose free)
Journal: BMC Genomic Data (2022) IF:
DOI: 10.1186/s12863-022-01070-w
Product Cited: RPMI-1640, DMEM(High glucose)