PM150210
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Product Introduction
Background
DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times.
The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucose type), which has been widely used in cell culture.
DMEM (High glucose) was widely used in fast growth, low adhesion cells, hybridoma myeloma cells, clone cells, DNA transfected transformation cells, various primary virus host cells, single cell culture and vaccine production.
DMEM (High glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 4500 mg/L D-Glucose
• 4 mM L-Glutamine
• 3700 mg/L NaHCO₃
• 15 mg/L Phenol red
• 1 mM Sodium pyruvate
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
This product is manufactured in a GMP-compliant factory, formulated with water for injection, and has obtained ISO 9001 and ISO 13485 certifications.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Medium | Components 4500 mg/L D-Glucose 4 mM L-Glutamine 3700 mg/L NaHCO₃ 1 mM Sodium Pyruvate 15 mg/L Phenol Red |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: Scientific Reports (2025) IF: 3.8
DOI: 10.1038/s41598-025-00909-3
Product Cited: DMEM (High glucose) Medium, RPMI-1640 Medium, Ham's F-12K Medium
Journal: Scientific Reports (2025) IF: 3.8
DOI: 10.1038/s41598-025-00747-3
Product Cited: NCI-H23 Cells Line, NCI-H1975 Cells Line, DMEM (High glucose) Medium, RPMI-1640 Medium, A549 [A-549] Cells Line, NCI-H460 [H460] Cells Line, Phosphate Buffer (PBS, 1 ×), BEAS-2B Cells Line, Fetal Bovine Serum, HCC4006 Cells Line, NCI-H1299 Cells Line
Journal: Scientific Reports (2025) IF: 3.8
DOI: 10.1038/s41598-025-01956-6
Product Cited: DMEM (High glucose) Medium
Journal: Scientific Reports (2025) IF: 3.8
DOI: 10.1038/s41598-025-00843-4
Product Cited: DMEM (High glucose) Medium
Journal: Scientific Reports (2025) IF: 3.8
DOI: 10.1038/s41598-025-00112-4
Product Cited: DMEM (High glucose) Medium