PM150220
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Product Introduction
Background
DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times.
The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucoser type), which has been widely used in cell culture.
DMEM (Low glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000 mg/L D-Glucose
• 4 mM L-Glutamine
• 3700 mg/L NaHCO₃
• 15 mg/L Phenol red
• 1 mM Sodium pyruvate
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: Tissue Engineering Part C-Methods (2025) IF: 2.7
DOI: 10.1089/ten.tec.2024.0298
Product Cited: DMEM (Low glucose) Medium
Journal: GENE (2025) IF: 2.6
DOI: 10.1016/j.gene.2025.149483
Product Cited: 100 mM Sodium Pyruvate Solution, 100 ×, Hela Cells Line, Fetal Bovine Serum, DMEM (High glucose) Medium, 293T [HEK-293T] Cells Line, 200 g/L D-Glucose Solution, 200 mM L-Glutamine Solution, 100 ×, Penicillin-Streptomycin Solution, 100 ×, Poly-L-Lysine Solution, 10 ×, DMEM (Low glucose) Medium
Journal: Materials Today Bio (2025) IF: 10.2
DOI: 10.1016/j.mtbio.2025.102023
Product Cited: DMEM (Low glucose) Medium
Journal: Cell Reports (2024) IF: 7.5
DOI: 10.1016/j.celrep.2024.115139
Product Cited: DMEM (Low glucose) Medium, Penicillin-Streptomycin-Amphotericin B Solution, 100 ×
Journal: Cell Death Discovery (2024) IF: 7.0
DOI: 10.1038/s41420-024-02030-2
Product Cited: Fetal Bovine Serum, DMEM (High glucose) Medium, DMEM (Low glucose) Medium