PM151010
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Product Introduction
Background
The buffer system of Leibovitz's L-15 medium is composed of phosphate and free amino acids, instead of the traditional sodium bicarbonate buffer system. Meanwhile, the substitution of galactose and sodium pyruvate for glucose prevents prevents the formation of acidic metabolic by-products, helps to maintain the stable pH of the medium. It is suitable for cell culture in a non-CO₂ balanced environment. Leibovitz's L-15 medium is suitable for the culture of monkey kidney cells and HEP-2, the isolation of primary cells (such as embryonic and adult tissues), the culture of various viruses and the culture of neurons.
Leibovitz's L-15 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 300 mg/L L-Glutamine
• 10 mg/L Phenol red
• 900 mg/L D-galactose
• 550 mg/L Sodium pyruvate
• Phosphate buffer system Balanced salt
Without
• D-Glucose
• HEPES
• NaHCO₃
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: Frontiers in Bioscience-Landmark (2023) IF: 3.1
Product Cited: DMEM(High glucose), Leibovitz's L-15, Ham's F-12K, Fetal Bovine Serum, Annexin V-FITC Reagent, DMEM(High glucose)
Journal: Chinese Journal of Integrative Medicine (2023) IF: 2.9
DOI: 10.1007/s11655-023-3698-9
Product Cited: Caco-2 Cells Line, HCT 116 Cells Line, HT-29 Cells Line, SW480 [SW-480] Cells Line, MEM, McCoy's 5A, Leibovitz's L-15
Journal: HEREDITAS (2023) IF: 2.700
DOI: 10.1186/s41065-023-00275-y
Product Cited: MDA-MB-231 Cells Line, MCF7 [MCF-7] Cells Line, 293T [HEK-293T] Cells Line, MCF 10A Cells Line, Leibovitz's L-15, MEM, DMEM/F12, DMEM(High glucose)
Journal: HORMONE AND METABOLIC RESEARCH (2023) IF: 2.2
DOI: 10.1055/a-2125-7018
Product Cited: Fetal Bovine Serum, RPMI-1640, IMDM, DMEM(High glucose), Leibovitz's L-15, Penicillin-Streptomycin Solution, 100×
Journal: Open Medicine (2023) IF: 2.100
Product Cited: MCF 10A Cells Line, MCF7 [MCF-7] Cells Line, MDA-MB-231 Cells Line, MDA-MB-436 Cells Line, SK-BR-3 [SKBR3] Cells Line, DMEM/F12, Donor Equine Serum, MEM Non-Essential Amino Acid Solution (NEAA), 100×, Penicillin-Streptomycin Solution, 100×, RPMI-1640 (ATCC Modification), Leibovitz's L-15, McCoy's 5A, Fetal Bovine Serum