PM150410
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Product Introduction
Background
MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells.
MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
• NEAA
• Earle's Balanced salt
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: Probiotics and Antimicrobial Proteins (2025) IF: 4.4
DOI: 10.1007/s12602-025-10516-3
Product Cited: HK-2 Cells Line, MEM, with NEAA Medium
Journal: FASEB JOURNAL (2025) IF: 4.4
Product Cited: Hacat Cells Line, MEM, with NEAA Medium
Journal: Frontiers in Pharmacology (2025) IF: 4.4
DOI: 10.3389/fphar.2024.1532610
Product Cited: MEM, with NEAA Medium, DMEM (Glucose free) Medium, U-2 OS [U2OS] Cells Line, McCoy's 5A Medium, HOS Cells Line
Journal: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2025) IF: 4.3
DOI: 10.1111/jcmm.70428
Product Cited: RPMI-1640 Medium, MEM, with NEAA Medium
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY (2025) IF: 4.2
DOI: 10.1016/j.ejphar.2025.177746
Product Cited: Fetal Bovine Serum, Hela Cells Line, MDA-MB-231 Cells Line, DMEM (High glucose) Medium, U-2 OS [U2OS] Cells Line, MEM, with NEAA Medium, Penicillin-Streptomycin Solution, 100 ×, McCoy's 5A Medium, MCF7 [MCF-7] Cells Line